SAFETY TESTS

The following safety tests were performed on the raw material '' Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40 ''.

- Cell toxicity (in-House Lab.)
- Bacterial Reverse Mutation Assay test (GLP Lab.)
- Chromosomal test (GLP Lab.)

The following additional tests are conducted on the safety of 'Methionyl r-Clostridium Botulinum Polypeptide-1 Hexapeptide-40' and animal testing of this ingredient is never carried out.

- Skin Irritation using EPISKIN
- Eye Irritation using EpiOcular test
- Skin Sensitization : Local Lymph node Assay : BrdU-ELISA)
- in vitro 3T3 NRU Photoxity Test

CLINICAL TESTS

Single Human Patch Test
Bolca Anti-Wrinkle Facial Serum
(I.E.C. Korea @ Chonnam National Univ. Hospital)

Clinical Test (I.E.C. Korea)
Bolca Anti-Wrinkle Facial Serum
(I.E.C. Korea)

D0

D28

Roughness

Net Elasticity

Nasolabial line

16/22

22/22

15/22

72 %

100 %

68 %

Ratio

Effectivenss

After 4 weeks test shows improvement of roughness, elasticity, nasolabial line from 70% of applicant. Different from individuals but the efficacy can be seen normally within 2 weeks